Electrophoresis
Electrophoresis
Pulling forces are equal
They don’t move at all
an ion of acid, breaks down to give minus, so attracted to the positive pole
And an ion of amine, breaks down to give plus, so it more attracted to the negative pole
The r group can have acid, or have an extra amine
When ion charge is unbalanced, the current pulls it one way
Electrophoresis
The blobs gets pulled by electrodes
The blobs moves by charge attraction
They move faster when charge is higher
Their size also affects their speed of movement
The bigger they are the clunkier they go
buffer pH lower, there is more protons, amines turns plus while acids stays constant
buffer pH higher, it is more basic, acids be minus but amines stays same